anti insulin pro insulin  (Progen Biotechnik)

Journal: Molecular Metabolism

Article Title: Beta-cell-specific C3 deficiency exacerbates metabolic dysregulation and insulin resistance in obesity

doi: 10.1016/j.molmet.2025.102302

Figure Lengend Snippet: A) Pancreas sections from treated mice were stained for glucagon (green) and insulin (red) to visualize islets and assess their size. Blue: DAPI. Scale bars, 50 μm. B) Quantification of total islet size in sections from 22 islets from pancreas sections of 7 LFD beta-C3-KO mice, 33 islets from pancreas sections of 5 HFD beta-C3-KO mice, and 35 islets from pancreas sections of 6 HFD C3-flox mice. C) Fraction of total islet staining for insulin, from the same islets analysed in (B). D) Examples of staining of individual HFD islets for glucagon (green) and pro-insulin (red). E) Quantification of pro-insulin staining in islets from each group of mice. F) Quantification of pro-insulin in serum of mice at given time points, measured by pro-insulin specific ELISA. G) Transmission electron micrographs of pancreatic islet cells isolated from beta-C3-KO or C3-flox mice. Beta-C3-KO mouse beta-cells contain accumulated amounts of swollen ER or similar vesicular organelles (black arrows), while normal ER is apparent in C3-flox mouse islet cells (white arrows). See also . Increased amounts of autophagic material were also observed in beta-C3-KO cells (asterisk). H) In vitro insulin secretion from isolated islets from C3-flox or beta-C3-KO mice HFD-fed mice, at 15 or 60 min. I) Average insulin content values per islet from secretion experiment in panel (H) (average values shown for each of 6 mice: from 6 wells, 5 islets per well). J) Serum C3 levels in C3-flox or beta-C3-KO mice at the endpoint of HFD, as measured by ELISA. Statistics in E, one-way ANOVA, in F/H, two-way ANOVA, in I, Student’s T-test, and in B/C, by mixed model statistical analysis (see methods).

Article Snippet: For pro-insulin staining, AlexaFluor647-labeled anti-pro-insulin (R&D Systems, #IC13361R, 1:100) was incubated overnight at 4 °C, in combination with glucagon staining, before washing and mounting as described above.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Transmission Assay, Isolation, In Vitro

Journal: Biology

Article Title: Among Other Tissues, Short-Term Garlic Oral Treatment Incrementally Improves Indicants of Only Pancreatic Islets of Langerhans Histology and Insulin mRNA Transcription and Synthesis in Diabetic Rats

doi: 10.3390/biology13050355

Figure Lengend Snippet: Effect of GE on insulin ( A , D ), pro-insulin ( B , E ), and C-peptide ( C , F ) in the bile duct and liver of diabetic rats. The immunohistochemical images of the bile duct and liver of DR-GE did not show any signs for insulin, pro-insulin, and C-peptide even after 8 weeks of treatment with the GE.

Article Snippet: Since only the pancreas revealed signs of insulin presence; thus, the co-localization of amylase (indicative of exocrine entities) and insulin (indicative of endocrine entities) was investigated by co-labeling the pancreatic tissue with rabbit anti-insulin/pro-insulin antibody (dilution ratio: 1:400—Invitrogen) and mouse anti-amylase (dilution ratio: 1:100—Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Immunohistochemical staining

Journal: Biology

Article Title: Among Other Tissues, Short-Term Garlic Oral Treatment Incrementally Improves Indicants of Only Pancreatic Islets of Langerhans Histology and Insulin mRNA Transcription and Synthesis in Diabetic Rats

doi: 10.3390/biology13050355

Figure Lengend Snippet: Effect of GE on insulin ( A , D ), pro-insulin ( B , E ), and C-peptide ( C , F ) in the bile duct and liver of diabetic rats. The immunohistochemical images of the bile duct and liver of DR-GE did not show any signs for insulin, pro-insulin, and C-peptide even after 8 weeks of treatment with the GE.

Article Snippet: Different tissue sections were incubated overnight with rabbit anti-insulin-1 antibody (dilution ratio: 1:400—Abcam, Cambridge, UK), rabbit anti-C-peptide antibody (dilution ratio: 1:100—Cell Signaling Technology, Danvers, MA, USA), or mouse anti-insulin/pro-insulin antibody (dilution ratio: 1:400—Invitrogen, Carlsbad, CA, USA) diluted in blocking buffer at 4 °C.

Techniques: Immunohistochemical staining